检索范围:
排序: 展示方式:
《医学前沿(英文)》 2023年 第17卷 第3期 页码 493-502 doi: 10.1007/s11684-022-0946-x
关键词: ALK fusion next-generation sequencing fluorescence in situ hybridization immunohistochemistry variant allele frequency intratumoral heterogeneity targeted therapy
基于荧光原位杂交技术的UASB颗粒污泥中苯酚降解微生物生态位分化研究 Article
Kengo Kubota, Kei Igarashi, Masayoshi Yamada, Yasuyuki Takemura, Yu-You Li, Hideki Harada
《工程(英文)》 2022年 第9卷 第2期 页码 61-66 doi: 10.1016/j.eng.2021.05.012
利用荧光原位杂交(FISH)和克隆文库构建技术,研究了厌氧污泥床反应器处理含酚废水时所收获的颗粒的微生物群落结构。研究发现,互营杆菌(Syntrophorhabdaceae)和隐杆厌氧菌(Cryptanaerobacter)的克隆是苯酚降解的主要原因。为了准确地对隐杆厌氧菌的克隆进行分类归属,需要利用近全长的16S核糖体RNA(rRNA)基因序列进行进化分析。设计了三种寡核苷酸探针用于检测以下三个分类群:互营杆菌、隐杆厌氧菌和互养菌。对厌氧颗粒薄片的FISH 分析显示细菌和古菌呈随机分布。然而,互营杆菌、隐杆厌氧菌和互养菌分布明确。在颗粒细胞的外层发现了隐杆厌氧菌和互养菌,两者关系密切,而互营杆菌则位于颗粒细胞的深层。细菌之间的代谢联系和对底物的亲和力很可能是造成这种特殊分布的原因。研究人员观察到颗粒污泥中苯酚的降解主要通过以下方式进行:首先,隐杆厌氧菌将苯酚转化为苯甲酸盐,然后被互养菌降解为乙酸盐。苯酚的这种互饲降解发生在颗粒表面附近,此处苯酚浓度较高。在苯酚浓度较低的颗粒深部,互营杆菌将苯酚降解为乙酸盐。结果表明,互营杆菌不太可能产生苯甲酸盐,并将其作为中间产物来喂养邻近的生物,这一点与以往研究所提出的理论相矛盾。
关键词: 隐杆厌氧菌(Cryptanaerobacter) 荧光原位杂交 苯酚厌氧降解 互营杆菌(Syntrophorhabdaceae) 互养菌(Syntrophus) 升流式厌氧污泥床(UASB)颗粒污泥
Gang GUO, Yayi WANG, Chong WANG, Hong WANG, Mianli PAN, Shaowei CHEN
《环境科学与工程前沿(英文)》 2013年 第7卷 第4期 页码 616-624 doi: 10.1007/s11783-013-0505-4
关键词: Denitrifying phosphorus removal anaerobic reaction time nitrous oxide polyhydroxyalkanoate free nitrous acid fluorescence in-situ hybridization
《化学科学与工程前沿(英文)》 2022年 第16卷 第1期 页码 92-102 doi: 10.1007/s11705-021-2063-9
关键词: hydrolysis reaction mitochondrial DNA damage in situ hydrophobic environment trigger fluorescence probe apoptosis
Nanqi REN, Han KANG, Xiuheng WANG, Nan LI
《环境科学与工程前沿(英文)》 2011年 第5卷 第2期 页码 277-282 doi: 10.1007/s11783-010-0226-x
关键词: fluorescent in situ hybridization (FISH) tetrad forming organisms (TFOs) temperature variation enhanced biological phosphorus removal (EBPR)
Ralf T. Voegele
《农业科学与工程前沿(英文)》 2017年 第4卷 第1期 页码 121-122 doi: 10.15302/J-FASE-2017142
Determination of heterozygosity for avirulence/virulence loci through sexual hybridization of
Yuan TIAN,Gangming ZHAN,Xia LU,Jie ZHAO,Lili HUANG,Zhensheng KANG
《农业科学与工程前沿(英文)》 2017年 第4卷 第1期 页码 48-58 doi: 10.15302/J-FASE-2016114
关键词: Puccinia striiformis f. sp. tritici selfing heterozygosity virulence inheritance
Effect of fiber hybridization on energy absorption and synergy in concrete
Ahmadreza RAMEZANI, Mohammad Reza ESFAHANI
《结构与土木工程前沿(英文)》 2019年 第13卷 第6期 页码 1338-1349 doi: 10.1007/s17009-019-0558-2
关键词: hybrid fiber-reinforced concrete synergy toughness steel fibers polypropylene fibers
Yulan GAO, Xiangchen FANG, Zhenmin CHENG
《化学科学与工程前沿(英文)》 2011年 第5卷 第3期 页码 287-296 doi: 10.1007/s11705-010-0529-2
关键词: ex-situ presulfurization in situ presulfurization hydrotreating catalyst sulfur utilization ratio
Xin ZHANG, Zichen LI
《化学科学与工程前沿(英文)》 2013年 第7卷 第4期 页码 381-387 doi: 10.1007/s11705-013-1359-9
Polyolefin-nanocomposites with special properties by in-situ polymerization
Walter Kaminsky
《化学科学与工程前沿(英文)》 2018年 第12卷 第3期 页码 555-563 doi: 10.1007/s11705-018-1715-x
Exceptionally strong polyolefin nanocomposites are synthesized by in-situ polymerization using soluble metallocene/methylaluminoxane (MAO) as catalysts in a two-step process. First, the soluble metallocene/MAO or other single site catalysts are absorbed on the surface of the nanofillers. Then by addition of ethene or propene, a polyolefin film is formed, covering the nanoparticles, layered silicates, or fibers. The resulting polyethylene and polypropylene nanocomposites are characterized by better physical and chemical properties such as stiffness, gas barrier properties, degradation temperatures, and crystallization rates. They show better mechanical properties than materials produced by mechanical blending. The thickness of the polyolefin can be controlled by the pressure of ethene or propene and by the polymerization time. Carbon fibers and carbon nanotubes are covered with isotactic or syndiotactic polypropylene. Because of the hydrophobic character of the carbon surface, the polymer is drawn on the fiber. This leads to a reinforced combined polymer with special properties. The crystallization temperature is 10 °C higher and therefore the crystallization rate up to 20 times faster than that of pure syndiotactic polypropylene. The form stability increases by 100% if 3 wt-% of carbon nanotubes are incorporated.
关键词: polyolefin nanocomposites metallocenes methylaluminoxane
ZHU Xiaoqin, QIAN Ying, LU Zhifeng
《化学科学与工程前沿(英文)》 2007年 第1卷 第4期 页码 381-384 doi: 10.1007/s11705-007-0069-6
关键词: 2-arylvinyl trans-vinylene character effective fluorescence push-core-pull-core-push
Carbon dots-based fluorescence sensor for two-photon imaging of pH in diabetic mice
《化学科学与工程前沿(英文)》 2023年 第17卷 第3期 页码 298-306 doi: 10.1007/s11705-022-2212-9
Muhammad Farooq Saleem Khan, Jing Wu, Cheng Cheng, Mona Akbar, Chuanyang Liu, Bo Liu, Jian Shen, Yu Xin
《环境科学与工程前沿(英文)》 2020年 第14卷 第3期 doi: 10.1007/s11783-020-1219-z
关键词: Fluorescence Photophysical properties Effect of the substituent Toxic aromatic compounds
Xia Hou, Liping Huang, Peng Zhou, Hua Xue, Ning Li
《环境科学与工程前沿(英文)》 2018年 第12卷 第4期 doi: 10.1007/s11783-018-1057-4
Cell membrane of indigenous Cd-tolerant EAB harbored more cadmium than chromium. Indigenous Cd-tolerant EAB cytoplasm located more chromium than cadmium. Simultaneously quantitatively imaging Cd(II) and Cr(III) ions in EAB was achieved. Current accelerated the harboring of cadmium in EAB at an initial 2 h. Current directed the accumulation of more chromium in EAB over time.
关键词: Microbial electrolysis cell Electrochemically active bacteria Cd-tolerant bacteria Cd(II) and Cr(VI) Fluorescence probe
标题 作者 时间 类型 操作
Potential unreliability of ALK variant allele frequency in the efficacy prediction of targeted therapy in NSCLC
期刊论文
基于荧光原位杂交技术的UASB颗粒污泥中苯酚降解微生物生态位分化研究
Kengo Kubota, Kei Igarashi, Masayoshi Yamada, Yasuyuki Takemura, Yu-You Li, Hideki Harada
期刊论文
Short-term effects of excessive anaerobic reaction time on anaerobic metabolism of denitrifying polyphosphate- accumulating organisms linked to phosphorus removal and N
Gang GUO, Yayi WANG, Chong WANG, Hong WANG, Mianli PAN, Shaowei CHEN
期刊论文
hydrophobic environment triggering reactive fluorescence probe to real-time monitor mitochondrial DNA
期刊论文
Short-term effect of temperature variation on the competition between PAOs and GAOs during acclimation period of an EBPR system
Nanqi REN, Han KANG, Xiuheng WANG, Nan LI
期刊论文
Comments on “Determination of heterozygosity for avirulence/virulence loci through sexual hybridization
Ralf T. Voegele
期刊论文
Determination of heterozygosity for avirulence/virulence loci through sexual hybridization of
Yuan TIAN,Gangming ZHAN,Xia LU,Jie ZHAO,Lili HUANG,Zhensheng KANG
期刊论文
Effect of fiber hybridization on energy absorption and synergy in concrete
Ahmadreza RAMEZANI, Mohammad Reza ESFAHANI
期刊论文
Development and application of ex-situ presulfurization technology for hydrotreating catalysts in China
Yulan GAO, Xiangchen FANG, Zhenmin CHENG
期刊论文
Synthesis and fluorescence behavior of 2,5-diphenyl-1,3,4-oxadiazole-containing bismaleimides and bissuccinimides
Xin ZHANG, Zichen LI
期刊论文
Synthesis and characterization of 2,5-bis[4-(2-arylvinyl)phenyl]-1,3,4-oxadiazoles with two-photon fluorescence
ZHU Xiaoqin, QIAN Ying, LU Zhifeng
期刊论文
Insight into fluorescence properties of 14 selected toxic single-ring aromatic compounds in water: Experimental
Muhammad Farooq Saleem Khan, Jing Wu, Cheng Cheng, Mona Akbar, Chuanyang Liu, Bo Liu, Jian Shen, Yu Xin
期刊论文